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Product Name
LRP5 antibody
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Description
LRP5 Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue, human hepat℃irrhosis tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 200 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
BMND1 antibody; EVR1 antibody; EVR4 antibody; HBM antibody; LR3 antibody; LRP 5 antibody; LRP5 antibody; LRP7 antibody; OPPG antibody; OPS antibody; OPTA1 antibody; VBCH2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LRP5 recombinant protein (Accession Number: NM_002335). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112323(LRP5 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:112323(LRP5 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:112323(LRP5 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:112323(LRP5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Low-density lipoprotein receptor-related protein 5 (LRP5) is a single-pass type I membrane protein with EGF-like and LDLR domains in the extracellular domain. LRP5 and LRP6 act as coreceptors with Frizzled for Wnt. The complex of Wnt-Fzd-LRP5-LRP6 triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. LRP5 plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in LRP5 gene.
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