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Product Name
LMP7 antibody
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Description
LMP7 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive FC detected in HepG2 cells. Positive WB detected in mouse kidney tissue, human kidney tissue, Jurkat cells, Raji cells. Positive IP detected in Raji cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
beta5i antibody; D6S216 antibody; D6S216E antibody; LMP7 antibody; Low molecular mass protein 7 antibody; Macropain subunit C13 antibody; Proteasome component C13 antibody; Proteasome subunit beta 5i antibody; Proteasome subunit beta type 8 antibody; PSMB5i antibody; PSMB8 antibody; RING10 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LMP7 recombinant protein (Accession Number: NM_004159). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:112287(PSMB8 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112287(PSMB8 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112287(PSMB8 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PSMB8 antibody Catalog No:112287 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PSMB8 (IP:Catalog No:112287, 3ug; Detection:Catalog No:112287 1:1000) with Raji cells lysate 2800ug.
1X10^6 HepG2 cells were stained with 0.2ug PSMB8 antibody (Catalog No:112287, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).
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