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Product Name
Anti-Proteasome 20S LMP7 Rabbit antibody
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Description
Proteasome 20S LMP7 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human LMP7
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
IHC: 1/50
ICC/IF: 1/20
FC: 1/20
IP: 1/20
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Validations
Western blot detection of LMP7 in 3T3,Hela cell lysates using LMP7 Rabbit pAb(1:1000 diluted).Predicted band size:30kDa.Observed band size:23kDa.
Immunocytochemistry of LMP7(green) in Hela cells using LMP7 Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.P28062.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"