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Product Name
liver Arginase antibody
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Description
liver Arginase Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, human colon tissue, human heart tissue, human liver tissue, mouse heart tissue. Positive IP detected in mouse liver tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue, human heart tissue, human kidney tissue, human liver cancer tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue, mouse kidney tissue, mouse liver tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 35-36 kDa
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Tested applications
ELISA, IHC, IP, IF, FC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Arginase 1 antibody; liver Arginase antibody; Liver type arginase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of liver Arginase recombinant protein (Accession Number: BC005321). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:112248(ARG1 antibody) at dilution of 1:1000
IP Result of anti-ARG1 (IP:Catalog No:112248, 3ug; Detection:Catalog No:112248 1:1000) with mouse liver tissue lysate 12500ug.
Immunohistochemical of paraffin-embedded human liver using Catalog No:112248(ARG1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:112248(ARG1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112248(ARG1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug ARG1 antibody (Catalog No:112248, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Liver arginase(Arginase-1) belongs to the arginase family. Hepatic arginase 1 is closely associated with alternative macrophage activation(PMID:12098359) and Arg1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN).
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References
- Yu K, Yang J, Wang F. Ethanol supports macrophage recruitment and reinforces invasion and migration of Lewis lung carcinoma. Alcoholism, clinical and experimental research. 38(10):2597-606. 2014.
- Mukhopadhyay D, Mukherjee S, Roy S. M2 Polarization of Monocytes-Macrophages Is a Hallmark of Indian Post Kala-Azar Dermal Leishmaniasis. PLoS neglected tropical diseases. 9(10):e0004145. 2015.
- Yang J, Qi F, Gu H. Neonatal BCG vaccination of mice improves neurogenesis and behavior in early life. Brain research bulletin. 120:25-33. 2016.
- Qi F, Yang J, Xia Y. A(H1N1) vaccination recruits T lymphocytes to the choroid plexus for the promotion of hippocampal neurogenesis and working memory in pregnant mice. Brain, behavior, and immunity. 2015.
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