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Product Name
Liprin alpha 1 antibody
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Description
Liprin alpha 1 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human prostate cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, MCF7 cells. Positive IP detected in MCF-7 cells. Observed molecular weight by Western-blot: 160 kDa
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Tested applications
ELISA, WB, IHC, IF, IP, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ41337 antibody; FLJ42630 antibody; FLJ43474 antibody; LAR interacting protein 1 antibody; LIP 1 antibody; LIP.1 antibody; LIP1 antibody; LIPRIN antibody; Liprin alpha 1 antibody; PPFIA1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Liprin alpha 1 recombinant protein (Accession Number: NM_003626). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112245(PPFIA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112245(PPFIA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112245(PPFIA1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PPFIA1 antibody Catalog No:112245 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PPFIA1 (IP:Catalog No:112245, 4ug; Detection:Catalog No:112245 1:500) with MCF-7 cells lysate 3200ug.
1X10^6 HeLa cells were stained with 0.2ug PPFIA1 antibody (Catalog No:112245, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.
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References
- Astro V, Chiaretti S, Magistrati E, Fivaz M, de Curtis I. Liprin-α1, ERC1 and LL5 define polarized and dynamic structures that are implicated in cell migration. Journal of cell science. 127(Pt 17):3862-76. 2014.
- Chiaretti S, Astro V, Chiricozzi E, de Curtis I. Effects of the scaffold proteins liprin-α1, β1 and β2 on invasion by breast cancer cells. Biology of the cell / under the auspices of the European Cell Biology Organization. 108(3):65-75. 2016.
- Choi EJ, Yun JA, Jabeen S. Prognostic significance of TMEM16A, PPFIA1, and FADD expression in invasive ductal carcinoma of the breast. World journal of surgical oncology. 12:137. 2014.
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