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Product Name
LIMK1 antibody
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Description
LIMK1 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human brain tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 73 kDa,33 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
LIM domain kinase 1 antibody; LIMK antibody; LIMK 1 antibody; LIMK1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002314). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112228(LIMK1 antibody) at dilution of 1:1000
IP Result of anti-LIMK1 (IP:Catalog No:112228, 4ug; Detection:Catalog No:112228 1:800) with HeLa cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:112228(LIMK1 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:112228(LIMK1 Antibody) at dilution of 1:200 (under 40x lens)
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Background
LIMK1,also named LIMK, belongs to the protein kinase superfamily and TKL Ser/Thr protein kinase family. LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. LIMK1 may be involved in brain development. The antibody has no cross-reaction with LIMK2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"