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Product Name
LIG3 Polyclonal Antibody
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Description
Polyclonal antibody to LIG3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
LIG3 antibody; LIG2 antibody; DNA ligase 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human LIG3 (NP_039269.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - LIG3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using LIG3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - LIG3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using LIG3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - LIG3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human oophoroma using LIG3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - LIG3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using LIG3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - LIG3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using LIG3 antibody at dilution of 1:100 (40x lens).
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Background
Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"