Cart 2

LIFR antibody

Cat.#: 112221

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    LIFR antibody
  • Documents
  • Description
    LIFR Rabbit Polyclonal antibody. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in human skeletal muscle tissue, mouse skeletal muscle tissue. Positive FC detected in HeLa cells. Positive IHC detected in human skeletal muscle tissue, human kidney tissue, human placenta tissue. Observed molecular weight by Western-blot: 190 kDa
  • Tested applications
    ELISA, WB, IHC, IP, FC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CD118 antibody; FLJ98106 antibody; FLJ99923 antibody; LIF R antibody; LIF receptor antibody; LIFR antibody; SJS2 antibody; STWS antibody; SWS antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of LIFR recombinant protein (Accession Number: XM_011514040). Purification method: Antigen Affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:112221(LIFR antibody) at dilution of 1:800

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:112221(LIFR antibody) at dilution of 1:800

    Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:112221(LIFR Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human skeletal muscle slide using Catalog No:112221(LIFR Antibody) at dilution of 1:50 (under 40x lens)

    IP Result of anti-LIFR (IP:Catalog No:112221, 4ug; Detection:Catalog No:112221 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

    IP Result of anti-LIFR (IP:Catalog No:112221, 4ug; Detection:Catalog No:112221 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

    1X10^6 HeLa cells were stained with .2ug LIFR antibody (Catalog No:112221, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 HeLa cells were stained with .2ug LIFR antibody (Catalog No:112221, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    LIFR, also known as CD118, is a subunit of a receptor for leukemia inhibitory factor (LIF). LIF is a pleiotropic cytokine of the interleukin-6 family which affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of LIF. The high-affinity complex also binds a related cytokine, oncostatin M (PMID: 8999038). LIFR has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway (PMID: 23001183).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"