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Product Name
LGR6 antibody
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Description
LGR6 Rabbit Polyclonal antibody. Positive WB detected in mouse testis tissue, human brain tissue, mouse ovary tissue, PC-3 cells. Positive IHC detected in human testis tissue, human ovary tissue, human skin tissue, human spleen tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 104 kDa
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Tested applications
ELISA, IHC, WB, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ14471 antibody; GPCR antibody; LGR6 antibody; VTS20631 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LGR6 recombinant protein (Accession Number: NM_021636). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:112212(LGR6 antibody) at dilution of 1:100
Immunohistochemical of paraffin-embedded human testis using Catalog No:112212(LGR6 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:112212(LGR6 antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug LGR6 antibody (Catalog No:112212, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) is a seven-transmembrane protein. LGR6 is a high affinity receptor of R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. It potentially functions as a tumor suppressor. (PMID: 20223988; 20417836; 22615920)
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