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Product Name
LEO1 antibody
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Description
LEO1 Rabbit Polyclonal antibody. Positive WB detected in mouse heart tissue, HeLa cells, HL-60 cells, human placenta tissue, Jurkat cells, mouse brain tissue, mouse skeletal muscle tissue. Positive IF detected in Hela cells. Positive IHC detected in human colon tissue. Observed molecular weight by Western-blot: 105kd
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
LEO1 antibody; RDL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LEO1 recombinant protein (Accession Number: NM_138792). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112196(LEO1 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using LEO1 antibody Catalog No:112196 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:112196(LEO1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon tissue slide using Catalog No:112196(LEO1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
LEO1 represents Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"