LC3A-Specific antibody

LC3A-Specific antibody

• DataSheet
• Material Safety Data Sheets (MSDS)

LC3A-Specific Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive FC detected in HeLa cells. Positive WB detected in mouse brain tissue, human brain tissue, mouse testis tissue. Observed molecular weight by Western-blot: 15-18kd

ELISA, WB, IF, FC

Human,Mouse,Rat; other species not tested.

LC3 antibody; LC3A antibody; MAP1A/MAP1B light chain 3 A antibody; MAP1ALC3 antibody; MAP1BLC3 antibody; MAP1LC3A antibody

Rabbit IgG

This antibody was obtained by immunization of Peptide (Accession Number: NM_032514). Purification method: Antigen affinity purified.

Polyclonal

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Store at -20℃. DO NOT ALIQUOT

LC3A, also named as MAP1LC3A, LC3, MAP1ALC3 and MAP1BLC3, belongs to the MAP1 LC3 family. LC3A is one of the light chain subunits and can associate with either MAP1A or MAP1B which are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. (PMID:11060023) MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.(PMID:12740394 ) This antibody is specific to LC3A.It recognize both LC3A-I and LC3A-II.

• Zhu X, Ruan Z, Yang X. Connexin 31.1 degradation requires the Clathrin-mediated autophagy in NSCLC cell H1299. Journal of cellular and molecular medicine. 19(1):257-64. 2015.