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Product Name
LBP antibody
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Description
LBP Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, A431 cells, A549 cells, HeLa cells, Jurkat cells, L02 cells, rat liver tissue. Positive IF detected in L02 cells, HepG2 cells. Positive IHC detected in human liver cancer tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 63-67 kDa
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Tested applications
ELISA, WB, IHC, FC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
LBP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LBP recombinant protein (Accession Number: BC022256). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:112161(LBP antibody) at dilution of 1:1000
Immunofluorescent analysis of L02 cells using Catalog No:112161(LBP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-LBP (IP:Catalog No:112161, 4ug; Detection:Catalog No:112161 1:1000) with mouse liver tissue lysate 4000ug.
1X10^6 HeLa cells were stained with 0.2ug LBP antibody (Catalog No:112161, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:112161(LBP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:112161(LBP antibody) at dilution of 1:50 (under 40x lens)
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Background
LBP belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family. It binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. LBP is involved in the acute-phase immunologic response to gram-negative bacterial infections. In many tissue/cells, we get 53-60kd and 63-67kd(Glycosylation form).
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