Product Name

LASP1 antibody

Documents

Description

LASP1 Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, MDA-MB-453s cells, mouse brain tissue, PC-3 cells, SGC-7901 cells. Positive IP detected in rat brain tissue. Positive IF detected in Hela cells. Positive IHC detected in human breast cancer tissue, human colon cancer tissue. Observed molecular weight by Western-blot: 38-40 kDa

Tested applications

ELISA, WB, IF, IHC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

Lasp 1 antibody; LASP1 antibody; LIM and SH3 domain protein 1 antibody; LIM and SH3 protein 1 antibody; MLN 50 antibody; MLN50 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of LASP1 recombinant protein (Accession Number: NM_006148). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:112152(LASP1 antibody) at dilution of 1:2000

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:112152(LASP1 antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of Hela cells, using LASP1 antibody Catalog No:112152 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-LASP1 (IP:Catalog No:112152, 4ug; Detection:Catalog No:112152 1:1500) with rat brain tissue lysate 4000ug.

Background

LASP1 represents LIM and SH3 protein 1

References

Zhang H, Li Z, Chu B. Upregulated LASP-1 correlates with a malignant phenotype and its potential therapeutic role in human cholangiocarcinoma. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016.
Li Z, Chen Y, Wang X. LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway. Cancer letters. 372(2):239-50. 2016.
Zeng Z, Bo H, Gong Z. AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.
Du YY, Zhao LM, Chen L. The tumor-suppressive function of miR-1 by targeting LASP1 and TAGLN2 in esophageal squamous cell carcinoma. Journal of gastroenterology and hepatology. 31(2):384-93. 2016.
Bo H, Gong Z, Zhang W. Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Oncotarget. 6(24):20404-18. 2015.

LASP1 antibody

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