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LAS1L antibody

Cat.#: 112151

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Product Information

  • Product Name
    LAS1L antibody
  • Documents
  • Description
    LAS1L Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HEK-293 cells, COLO 320 cells, HeLa cells, HepG2 cells, PC-3 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 83 kDa,70-75 kDa
  • Tested applications
    ELISA, WB, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    dJ475B7.2 antibody; FLJ12525 antibody; LAS1 like (S. cerevisiae) antibody; LAS1L antibody; Protein LAS1 homolog antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of LAS1L recombinant protein (Accession Number: NM_031206). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IF: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112151(LAS1L antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112151(LAS1L antibody) at dilution of 1:1000

    IP Result of anti-LAS1L (IP:Catalog No:112151, 4ug; Detection:Catalog No:112151 1:1000) with HEK-293 cells lysate 1200ug.

    IP Result of anti-LAS1L (IP:Catalog No:112151, 4ug; Detection:Catalog No:112151 1:1000) with HEK-293 cells lysate 1200ug.

    Immunofluorescent analysis of HepG2 cells using Catalog No:112151(LAS1L Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:112151(LAS1L Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    LAS1L is a nucleolar protein required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA. LAS1L interacts with PELP1, TEX10, and WDR18, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit.
  • References
    • Fanis P, Gillemans N, Aghajanirefah A. Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. Molecular & cellular proteomics : MCP. 11(11):1263-73. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"