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Product Name
LAMA4 antibody
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Description
LAMA4 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue. Positive WB detected in human heart tissue, human skeletal muscle tissue. Observed molecular weight by Western-blot: 11-13 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp686D23145 antibody; LAMA3 antibody; LAMA4 antibody; LAMA4* 1 antibody; Laminin 14 subunit alpha antibody; Laminin 8 subunit alpha antibody; Laminin 9 subunit alpha antibody; Laminin subunit alpha 4 antibody; laminin antibody; alpha 4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LAMA4 recombinant protein (Accession Number: NM_001105209). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:112269(LAMA4 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human ovary using Catalog No:112269(LAMA4 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:112269(LAMA4 antibody) at dilution of 1:100 (under 10x lens)
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Background
Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363).
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References
- Doppler K, Mittelbronn M, Lindner A, Bornemann A. Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis. Neuromuscular disorders : NMD. 19(6):406-11. 2009.
- Gutierrez-Roelens I, Galant C, Theate I. Rituximab treatment induces the expression of genes involved in healing processes in the rheumatoid arthritis synovium. Arthritis and rheumatism. 63(5):1246-54. 2011.
- Xu H, Hu F, Sado Y. Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone. Journal of neuroscience research. 86(7):1482-500. 2008.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"