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Product Name
KRT34 antibody
- Documents
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Description
KRT34 Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, A375 cells, A431 cells, HepG2 cells, L02 cells, mouse skin tissue. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Ha 4 antibody; HA4 antibody; Hair keratin antibody; type I Ha4 antibody; hHa4 antibody; HKA4 antibody; K34 antibody; keratin 34 antibody; Keratin antibody; type I cuticular Ha4 antibody; KRT34 antibody; KRTHA4 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KRT34 recombinant protein (Accession Number: NM_021013). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human skin using Catalog No:112131(KRT34 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin using Catalog No:112131(KRT34 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using KRT34 antibody Catalog No:112131 at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112131(KRT34 Antibody) at dilution of 1:600
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). KRT 34 is a type I keratin.
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