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Product Name
KRAS antibody
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Description
KRAS Mouse Monoclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human lung cancer tissue, human colon cancer tissue. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
C K RAS antibody; c Ki ras antibody; GTPase KRas antibody; K Ras 2 antibody; K RAS2A antibody; K RAS2B antibody; K RAS4A antibody; K RAS4B antibody; KI RAS antibody; KRAS antibody; KRAS1 antibody; KRAS2 antibody; KRAS2A antibody; KRAS2B antibody; KRAS2B speicifc antibody; Krasa antibody; Krasb antibody; NS3 antibody; RASK2 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of KRAS recombinant protein (Accession Number: XM_011520653). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107366(KRAS Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:107366(KRAS Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:107366(KRAS Antibody) at dilution of 1:50 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug KRAS antibody (Catalog No:107366, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, a and b.
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