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Product Name
KRAP, SSFA2 antibody
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Description
KRAP, SSFA2 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas tissue. Positive IP detected in mouse testis tissue. Positive WB detected in rat testis tissue. Observed molecular weight by Western-blot: 180 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
Cleavage signal 1 protein antibody; CS 1 antibody; CS1 antibody; DKFZp313O1039 antibody; DKFZp779G0129 antibody; FLJ45996 antibody; KIAA1927 antibody; KRAP antibody; SPAG13 antibody; sperm specific antigen 2 antibody; SSFA2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KRAP, SSFA2 recombinant protein (Accession Number: NM_006751). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
rat testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:112118(KRAP, SSFA2 antibody) at dilution of 1:600
IP Result of anti-KRAP, SSFA2 (IP:Catalog No:112118, 4ug; Detection:Catalog No:112118 1:500) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:112118(KRAP, SSFA2 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:112118(KRAP, SSFA2 Antibody) at dilution of 1:200 (under 40x lens).
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Background
KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)
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References
- Dingli F, Parys JB, Loew D, Saule S, Mery L. Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation. Journal of cell science. 125(Pt 22):5428-40. 2012.
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