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Product Name
KLHL12 antibody
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Description
KLHL12 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human testis tissue. Positive WB detected in PC-3 cells. Observed molecular weight by Western-blot: 53-56 kDa,63-67 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C3IP1 antibody; CUL3 interacting protein 1 antibody; DKIR antibody; FLJ27152 antibody; kelch like 12 (Drosophila) antibody; Kelch like protein 12 antibody; KLHL12 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KLHL12 recombinant protein (Accession Number: NM_021633). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112089(KLHL12 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:112089(KLHL12 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:112089(KLHL12 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:112089(KLHL12 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
KLHL12 represents kelch-like 12 (Drosophila)
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References
- Butkinaree C, Guo L, Ramkhelawon B. A regulator of secretory vesicle size, Kelch-like protein 12, facilitates the secretion of apolipoprotein B100 and very-low-density lipoproteins--brief report. Arteriosclerosis, thrombosis, and vascular biology. 34(2):251-4. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"