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Product Name
KL Polyclonal Antibody
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Description
Polyclonal antibody to KL
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
KL antibody; klotho antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human KL (NP_004786.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - KL Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using KL antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - KL Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using KL antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - KL Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human placenta using KL antibody at dilution of 1:200 (40x lens).
Immunofluorescence - KL Polyclonal Antibody
Immunofluorescence analysis of rat kidney using KL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KL Polyclonal Antibody
Immunofluorescence analysis of human placenta using KL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - KL Polyclonal Antibody
Immunofluorescence analysis of mouse kidney using KL antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"