Product Name

Kindlin 2 antibody

Documents

Description

Kindlin 2 Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive IHC detected in human ovary tumor. Positive WB detected in A549 cells, human placenta tissue. Positive IP detected in A549 cells. Observed molecular weight by Western-blot: 78kd

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DKFZp686G11125 antibody; Fermitin family homolog 2 antibody; FERMT2 antibody; FLJ34213 antibody; FLJ44462 antibody; KIND2 antibody; Kindlin 2 antibody; mig 2 antibody; MIG2 antibody; PLEKHC1 antibody; UNC112 antibody; UNC112B antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Kindlin 2 recombinant protein (Accession Number: NM_006832). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:112056(FERMT2 antibody) at dilution of 1:500

IP Result of anti-FERMT2 (IP:Catalog No:112056, 3ug; Detection:Catalog No:112056 1:1000) with A549 cells lysate 3300ug.

Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112056(FERMT2 antibody) at dilution of 1:50 (under 10x lens)

Immunofluorescent analysis of HepG2 cells, using FERMT2 antibody Catalog No:112056 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Background

FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.

References

Brahme NN, Harburger DS, Kemp-O'Brien K. Kindlin binds migfilin tandem LIM domains and regulates migfilin focal adhesion localization and recruitment dynamics. The Journal of biological chemistry. 288(49):35604-16. 2013.
Honda S, Shirotani-Ikejima H, Tadokoro S, Tomiyama Y, Miyata T. The integrin-linked kinase-PINCH-parvin complex supports integrin αIIbβ3 activation. PloS one. 8(12):e85498. 2013.
Huet-Calderwood C, Brahme NN, Kumar N. Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation. Journal of cell science. 127(Pt 19):4308-21. 2014.
Dowling JJ, Gibbs E, Russell M. Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function. Circulation research. 102(4):423-31. 2008.
Ji Y, Shen M, Wang X. Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins. Journal of proteome research. 11(6):3186-98. 2012.
Mahawithitwong P, Ohuchida K, Ikenaga N. Kindlin-2 expression in peritumoral stroma is associated with poor prognosis in pancreatic ductal adenocarcinoma. Pancreas. 42(4):663-9. 2013.
Feng C, Li YF, Yau YH. Kindlin-3 mediates integrin αLβ2 outside-in signaling, and it interacts with scaffold protein receptor for activated-C kinase 1 (RACK1). The Journal of biological chemistry. 287(14):10714-26. 2012.
Talaat S, Somji S, Toni C. Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer. Urology. 77(6):1507.e1-7. 2011.

Kindlin 2 antibody

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