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Product Name
Mouse KIRREL3/NEPH2 (His Tag) recombinant protein
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Description
Could be involved in the hematopoietic supportive capacity of stroma cells. The secreted extracellular domain is directly responsible for supporting hematopoietic stem cells.
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Protein name
Kin of IRRE-like protein 3
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Uniprot ID
Q8BR86
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Gene Name
Kirrel3; Kiaa1867
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse KIRREL3 (Q8BR86-1) extracellular domain (Met 1-Ala 535) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse KIRREL3 consists of 525 amino acids (pro form) and has a calculated molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant mouse KIRREL3 is approximately 65 kDa in SDS-PAGE under reducing conditions.
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Purity
> 96 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells.
When cells are added to mouse KIRREL3 coated plates (15 μg/mL, 100 μL/well), > 40% will adhere specifically after 90 minutes at 37 ℃. -
Validations
Mouse KIRREL3 / NEPH2 Protein (His Tag) SDS-PAGE
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