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Product Name
Anti-KIRREL3/NEPH2 antibody
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Description
Rabbit polyclonal to KIRREL3/NEPH2
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse KIRREL3 / NEPH2
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Alternative names
SST4 antibody; NEPH2 antibody; mKIAA1867 antibody; 1500010O20Rik antibody; 2900036G11Rik antibody; MRD4 antibody; KIRRE antibody; NEPH2 antibody; PRO4502 antibody; 1500010O20Rik antibody; 2900036G11Rik antibody; KIAA1867 antibody; KIRRE antibody; Kirrel3 antibody; KIRREL3 antibody; MGC126824 antibody; MGC126850 antibody; mKIAA1867 antibody; MRD4 antibody; NEPH2 antibody; NEPH2 antibody; PRO4502 antibody; SST4 antibody; UNQ5923/PRO4502/PRO19814 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse KIRREL3 / NEPH2 (rM KIRREL3 / NEPH2; Q8BR86-1; Met 1-Ala 535). KIRREL3 / NEPH2 specific IgG was purified by Mouse KIRREL3 / NEPH2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse KIRREL3. The detection limit for Mouse KIRREL3 is approximately 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
KIRREL3 / NEPH2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse KIRREL3 in mouse kidney with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
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Background
Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in fetalv and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the nucleogenesis of the PN through the control of neuronal migration inside the nucleus.
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References
- Bhalla K, et al. (2008) Alterations in CDH15 and KIRREL3 in patients with mild to severe intellectual disability. Am J Hum Genet. 83(6): 703-13.
- Gerke P, et al. (2005) NEPH2 is located at the glomerular slit diaphragm, interacts with nephrin and is cleaved from podocytes by metalloproteinases. J Am Soc Nephrol. 16(6): 1693-702.
- Gerke P, et al. (2006) Neuronal expression and interaction with the synaptic protein CASK suggest a role for Neph1 and Neph2 in synaptogenesis. J Comp Neurol. 498(4): 466-75.
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