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KDM3A,JMJD1A antibody

Cat.#: 112025

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Product Information

  • Product Name
    KDM3A,JMJD1A antibody
  • Documents
  • Description
    KDM3A,JMJD1A Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human kidney tissue, human ovary tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 150 kDa
  • Tested applications
    ELISA, IF, IP, WB, IHC
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    DKFZp686A24246 antibody; DKFZp686P07111 antibody; JHDM2A antibody; JHMD2A antibody; JMJD1 antibody; JMJD1A antibody; KDM3A antibody; KIAA0742 antibody; lysine (K) demethylase 3A antibody; Lysine specific demethylase 3A antibody; TSGA antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of KDM3A,JMJD1A recombinant protein (Accession Number: BC038297). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112025(KDM3A,JMJD1A antibody) at dilution of 1:2000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112025(KDM3A,JMJD1A antibody) at dilution of 1:2000

    IP Result of anti-KDM3A,JMJD1A (IP:Catalog No:112025, 5ug; Detection:Catalog No:112025 1:1000) with HeLa cells lysate 2500ug.

    IP Result of anti-KDM3A,JMJD1A (IP:Catalog No:112025, 5ug; Detection:Catalog No:112025 1:1000) with HeLa cells lysate 2500ug.

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112025(KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:112025(KDM3A,JMJD1A antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using KDM3A antibody Catalog No:112025 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using KDM3A antibody Catalog No:112025 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Background
    KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC. (PMID:20823141) This antibody recognize the N-term of KDM3A(JMJD1A).
  • References
    • Uemura M, Yamamoto H, Takemasa I. Jumonji domain containing 1A is a novel prognostic marker for colorectal cancer: in vivo identification from hypoxic tumor cells. Clinical cancer research : an official journal of the American Association for Cancer Research. 16(18):4636-46. 2010.
    • Oh HS, Bryant KF, Nieland TJ. A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression. mBio. 5(1):e01086-13. 2014.
    • Kasioulis I, Syred HM, Tate P. Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis. Molecular biology of the cell. 25(8):1216-33. 2014.
    • Ueda J, Ho JC, Lee KL. The hypoxia-inducible epigenetic regulators Jmjd1a and G9a provide a mechanistic link between angiogenesis and tumor growth. Molecular and cellular biology. 34(19):3702-20. 2014.
    • Yamada D, Kobayashi S, Yamamoto H. Role of the hypoxia-related gene, JMJD1A, in hepatocellular carcinoma: clinical impact on recurrence after hepatic resection. Annals of surgical oncology. 19 Suppl 3:S355-64. 2012.
    • Du ZM, Hu LF, Wang HY. Upregulation of MiR-155 in nasopharyngeal carcinoma is partly driven by LMP1 and LMP2A and downregulates a negative prognostic marker JMJD1A. PloS one. 6(4):e19137. 2011.
    • Cho HS, Toyokawa G, Daigo Y. The JmjC domain-containing histone demethylase KDM3A is a positive regulator of the G1/S transition in cancer cells via transcriptional regulation of the HOXA1 gene. International journal of cancer. 131(3):E179-89. 2012.
    • Kim KY, Huerta SB, Izumiya C. Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen regulates the KSHV epigenome by association with the histone demethylase KDM3A. Journal of virology. 87(12):6782-93. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"