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Product Name
IVD antibody
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Description
IVD Mouse Monoclonal antibody. Positive WB detected in Hela cells, HEK-293 cells, human liver tissue, Jurkat cells, MCF7 cells, Transfected HEK-293 cells. Positive IHC detected in human liver tissue. Positive IF detected in Hela cells, MCF-7 cells. Observed molecular weight by Western-blot: 43kD
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ACAD2 antibody; IVD antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of IVD recombinant protein (Accession Number: NM_002225). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107356(IVD antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107356(IVD antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107356(IVD antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using IVD antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107356(IVD Antibody) at dilution of 1:1000
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Background
IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.
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References
- Liu H, Zhang H, Cui R, Guo X, Wang D, Dai J. Activation of peroxisome proliferator-activated receptor α ameliorates perfluorododecanoic acid-induced production of reactive oxygen species in rat liver. Archives of toxicology. 2015.
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