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Product Name
Anti-Isovaleryl-CoA dehydrogenase / IVD antibody
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Description
Mouse monoclonal to Isovaleryl-CoA dehydrogenase / IVD
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Tested applications
ELISA, WB, IF, ICC/IF
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Species reactivity
Human Isovaleryl-CoA dehydrogenase / IVD
No cross-reactivity in ELISA with Insect cell lysate -
Alternative names
ACAD2 antibody; AI463340 antibody; 1300016K07Rik antibody; 6720455E18Rik antibody; mitochondrial antibody; 1300016K07Rik antibody; 6720455E18Rik antibody; mitochondrial antibody; ACAD2 antibody; AI463340 antibody; isovaleryl CoA dehydrogenase antibody; isovaleryl coenzyme A dehydrogenase antibody; isovaleryl Coenzyme A dehydrogenase antibody; isovaleryl dehydrogenase antibody; isovaleryl-CoA dehydrogenase antibody; IVD antibody; Ivd antibody; RP23-172B16.1 antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Isovaleryl-CoA dehydrogenase / IVD (rh Isovaleryl-CoA dehydrogenase / IVD; AAH17202.1; His30-His423). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human Isovaleryl-CoA dehydrogenase / IVD. The detection limit for Human Isovaleryl-CoA dehydrogenase / IVD is approximately 0.078 ng/well.
ICC/IF: 10-25 μg/mL
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Validations
Isovaleryl-CoA dehydrogenase / IVD Antibody, Mouse MAb, Western blot
Isovaleryl-CoA dehydrogenase / IVD Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human IVD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IVD monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Isovaleryl-CoA dehydrogenase / IVD Antibody, Mouse MAb, Western blot
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Background
Isovaleryl-CoA dehydrogenase, also known as IVD, plays an essential role in processing proteins obtained from the diet. The body breaks down proteins from food into smaller parts called amino acids. Amino acids can be further processed to provide energy for growth and development. Isovaleryl-CoA dehydrogenase helps process a particular amino acid called leucine. Specifically, isovaleryl-CoA dehydrogenase is responsible for the third step in the breakdown of leucine. This step is a chemical reaction that converts a molecule called isovaleryl-CoA to another molecule, 3-methylcrotonyl-CoA. Additional chemical reactions convert 3-methylcrotonyl-CoA into molecules that are used for energy.
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References
- BACHHAWAT BK, et al. (1956) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.
- Ikeda Y, et al. (1983) Purification and characterization of isovaleryl coenzyme A dehydrogenase from rat liver mitochondria. J Biol Chem. 258(2):1077-85.
- Tanaka K, et al. (1966) Enzymatic carboxylation of beta-hydroxyisovaleryl coenzyme A. J Biol Chem. 219(2):539-50.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"