ITPA antibody

Cat.#: 111955

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Product Information

  • Product Name
    ITPA antibody
  • Documents
  • Description
    ITPA Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human heart tissue, human lung cancer tissue. Positive IP detected in mouse liver tissue. Positive WB detected in K-562 cells, HeLa cells, HT-1080 cells, human liver tissue, mouse heart tissue, mouse liver tissue, NIH/3T3 cells, rat liver tissue. Observed molecular weight by Western-blot: 22 kDa
  • Tested applications
    ELISA, WB, IF, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    C20orf37 antibody; dJ794I6.3 antibody; HLC14 06 P antibody; Inosine triphosphatase antibody; ITPA antibody; ITPase antibody; OK/SW cl.9 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ITPA recombinant protein (Accession Number: NM_033453). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111955(ITPA antibody) at dilution of 1:1000

    K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:111955(ITPA antibody) at dilution of 1:1000

    Immunofluorescent analysis of MCF-7 cells, using ITPA antibody Catalog No:109111 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of MCF-7 cells, using ITPA antibody Catalog No:109111 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-ITPA (IP:Catalog No:111955, 4ug; Detection:Catalog No:111955 1:1000) with mouse liver tissue lysate 4000ug.

    IP Result of anti-ITPA (IP:Catalog No:111955, 4ug; Detection:Catalog No:111955 1:1000) with mouse liver tissue lysate 4000ug.

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:111955(ITPA Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:111955(ITPA Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:111955(ITPA Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:111955(ITPA Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    ITPA(Inosine triphosphate pyrophosphatase) catalyzes the pyrophosphohydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP).It expresses in the cytoplasm(PMID:11278832).It can be detected a band of 44 Kda as a homodimer in the western blot(PMID:19631656) though its predictedsize is 21kd.This protein has two isforms with the molecular weight of 20KDa and 21KDa.
  • References
    • Herting G, Barber K, Zappala MR, Cunningham RP, Burgis NE. Quantitative in vitro and in vivo characterization of the human P32T mutant ITPase. Biochimica et biophysica acta. 1802(2):269-74. 2010.
    • Gall AD, Gall A, Moore AC. Analysis of human ITPase nucleobase specificity by site-directed mutagenesis. Biochimie. 95(9):1711-21. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"