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Product Name
ITGA2 Polyclonal Antibody
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Description
Polyclonal antibody to ITGA2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ITGA2 antibody; BR antibody; CD49B antibody; GPIa antibody; HPA-5 antibody; VLA-2 antibody; VLAA2 antibody; integrin alpha-2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human ITGA2
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - ITGA2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ITGA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Western blot - ITGA2 Polyclonal Antibody
Western blot analysis of extracts from normal (control) and ITGA2 knockout HeLa cells, using ITGA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ITGA2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ITGA2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ITGA2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ITGA2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A.; (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"