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Product Name
IRGM Polyclonal Antibody
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Description
Polyclonal antibody to IRGM
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
IRGM antibody; IFI1 antibody; IRGM1 antibody; LRG-47 antibody; LRG47 antibody; immunity related GTPase M antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human IRGM
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:20 - 1:50 -
Validations
Western blot - IRGM Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IRGM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"