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Product Name
Human IL-4R/CD124 (His Tag) recombinant protein
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Description
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
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Protein name
Interleukin-4 receptor subunit alpha
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Protein short names
IL4R; MGC118473; CD124; IL4RA
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Uniprot ID
P24394
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Gene Name
IL4R; IL4RA; 582J2.1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human IL4R (NP_000409.1) precursor (Met 1-His 232) was fused with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The secreted recombinant human IL4R consists of 218 amino acids after cleavage of the signal peptide and predictes a molecular mass of 25.3 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 43-48 kDa due to glycosylation.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
1. Measured by its binding ability in a functional ELISA. Immobilized human IL4R-His (Cat:502315) at 10 μg/mL (100 μl/well) can bind biotinylated human IL4 (Cat:500224). The EC50 of biotinylated human IL4 is 20.8-48.5 ng/mL.
2. Measured by its ability to inhibit IL4 dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-4 ng/ml in the presence of 0.2 ng/ml Recombinant Human IL4. -
Validations
Human IL4R / CD124 Protein (His Tag) SDS-PAGE
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