Human IL-28B/IFN-lambda-3 (His Tag) recombinant protein

Human IL-28B/IFN-lambda-3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

Interferon lambda-3

IFNL3; IFL-1; IL28C; IL28B; INF-ALPHA; IFN-LAMBDA-3; IL-28B; INF-LAMBDA; IL28

Q8IZI9

IFNL3; IL28B; IL28C; ZCYTO22

Human Cells

A DNA sequence encoding the human IL28B (NP_742151.2) (Met 1-Val 196) was expressed, with a polyhistidine tag at the C-terminus.

Human

The recombinant human IL28B consists of 186 amino acids and predictes a molecular mass of 21 kDa. In SDS-PAGE under reducing conditions, rhIL28B migrates as an approximately 23 kDa band.

> 96 % as determined by SDS-PAGE

1. Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV). The EC₅₀ for this effect is typically 2-6 ng/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized human IL28B at 20 μg/ml (100 μl/well) can bind human IL10RB with a linear ranger of 3.125-25 μg/ml.