Rat IFNA4 (Fc Tag) recombinant protein

Rat IFNA4 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

Interferon, alpha 4 (Predicted)

RP23-139P14.30; MGC142200; IFNA4; MGC143607; IFA4; MUIFN-ALPHA 4

D3ZFH0

Ifna4; Ifna4_predicted; rCG_23012

Human Cells

A DNA sequence encoding the rat IFNA4 (D3ZFH0) (Cys24-Lys189) was expressed, fused with Fc region of human IgG1 at the N-terminus.

Rat

The recombinant rat IFNA4 comprises 426 amino acids and predicts a molecular mass of 47.5 kDa. The apparent molecular mass of the recombinant protein is approximately 48 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV).
The ED₅₀ for this effect is typically 0.5-4 μg/mL.