Mouse IFNA4 (Fc Tag) recombinant protein

Mouse IFNA4 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interferon, alpha 4 (IFNA4) belongs to the alpha/beta interferon family. Two variants of IFNA4 (IFNA4a and IFNA4b) are known, which differ from each other by changes in their coding regions at nucleotide positions 220 and 410 and can be distinguished by selective restriction enzyme analysis. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

Interferon alpha-4

IFA4; MGC143607; MUIFN-ALPHA 4; IFNA4; MGC142200; RP23-139P14.30

Q540C2

Ifna4

Human Cells

A DNA sequence encoding the extracellular domain of mouse IFNA4 (NP_034634.1) (Cys 25-Glu 186) was fused with the Fc region of human IgG1 at the N-terminus.

Mouse

The recombinant mouse IFNA4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 422 amino acids and has a calculated molecular mass of 47.2 kD as estimated in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV).
The ED₅₀ for this effect is typically 0.2-1 ng/mL.