Integrin beta-6-Specific antibody

Cat.#: 111866

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Product Information

  • Product Name
    Integrin beta-6-Specific antibody
  • Documents
  • Description
    Integrin beta-6-Specific Rabbit Polyclonal antibody. Positive WB detected in A549 cells, A2780 cells. Positive IHC detected in human lung tissue. Positive IF detected in A549 cells. Observed molecular weight by Western-blot: 97 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    Integrin beta 6 antibody; integrin antibody; beta 6 antibody; ITGB6 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Peptide (Accession Number: NM_000888). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:50-1:500

  • Validations

    A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:111866(ITGB6-Specific antibody) at dilution of 1:500

    A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:111866(ITGB6-Specific antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human lung using Catalog No:111866(ITGB6-Specific antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung using Catalog No:111866(ITGB6-Specific antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung using Catalog No:111866(ITGB6-Specific antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human lung using Catalog No:111866(ITGB6-Specific antibody) at dilution of 1:100 (under 40x lens)

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using Catalog No:111866(Integrin beta-6-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using Catalog No:111866(Integrin beta-6-Specific Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. The antibody is specific to ITGB6.
  • References
    • Niu Z, Wang J, Muhammad S. Protein expression of eIF4E and integrin αvβ6 in colon cancer can predict clinical significance, reveal their correlation and imply possible mechanism of interaction. Cell & bioscience. 4:23. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"