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Product Name
iNOS Polyclonal Antibody
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Description
Polyclonal antibody to iNOS
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Tested applications
WB, IHC, IF, FC
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Species reactivity
Human
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Alternative names
NOS2 antibody; HEP-NOS antibody; INOS antibody; NOS antibody; NOS2A antibody; nitric oxide synthase 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human iNOS
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:50
FC 1:20 - 1:50 -
Validations
Western blot - iNOS Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using iNOS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"