-
Product Name
Inhibin beta A antibody
- Documents
-
Description
Inhibin beta A Rabbit Polyclonal antibody. Positive WB detected in A549 cells, BGC-823 cells, HeLa cells, HepG2 cells, MCF7 cells, SGC-7901 cells. Positive IF detected in A549 cells, Hela cells. Positive IHC detected in human lung cancer tissue, human placenta tissue, human prostate cancer tissue. Observed molecular weight by Western-blot: 47 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
Activin beta A chain antibody; EDF antibody; FRP antibody; INHBA antibody; Inhibin beta A chain antibody; Inhibin β-A antibody; inhibin antibody; beta A antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Inhibin beta A recombinant protein (Accession Number: XM_017012176). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:111799(INHBA antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:111799(INHBA antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:111799(INHBA antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of A549 cells using Catalog No:111799(INHBA Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Inhibin is a 32-kD dimeric glycoprotein consisting of an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin βA forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin βA can also bind the βB and α isoforms to form activin AB and inhibin, respectively.
-
References
- Kashyap MK, Pawar HA, Keerthikumar S. Evaluation of protein expression pattern of stanniocalcin 2, insulin-like growth factor-binding protein 7, inhibin beta A and four and a half LIM domains 1 in esophageal squamous cell carcinoma. Cancer biomarkers : section A of Disease markers. 12(1):1-9. 2013.
- Cai K, Hua G, Ahmad S. Action mechanism of inhibin α-subunit on the development of Sertoli cells and first wave of spermatogenesis in mice. PloS one. 6(10):e25585. 2011.
- Dutta DJ, Zameer A, Mariani JN. Combinatorial actions of Tgfβ and Activin ligands promote oligodendrocyte development and CNS myelination. Development (Cambridge, England). 141(12):2414-28. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"