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Product Name
ILK Polyclonal Antibody
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Description
Polyclonal antibody to ILK
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ILK antibody; HEL-S-28 antibody; ILK-1 antibody; ILK-2 antibody; P59 antibody; p59ILK antibody; integrin linked kinase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ILK (NP_001014795.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - ILK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ILK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - ILK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using ILK Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ILK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ILK Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ILK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using ILK Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - ILK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using ILK Antibody at dilution of 1:200 (40x lens).
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Background
Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"