-
Product Name
IKZF3 antibody
- Documents
-
Description
IKZF3 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human spleen tissue. Positive WB detected in HepG2 cells, Jurkat cells. Observed molecular weight by Western-blot: 58-70kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
AIO antibody; AIOLOS antibody; IKZF3 antibody; Zinc finger protein Aiolos antibody; ZNFN1A3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of IKZF3 recombinant protein (Accession Number: NM_012481). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:111650(IKZF3 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human spleen using Catalog No:111650(IKZF3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:111650(IKZF3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody Catalog No:111650 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"