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Product Name
IKZF1 Polyclonal Antibody
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Description
Polyclonal antibody to IKZF1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
IKZF1 antibody; CVID13 antibody; Hs.54452 antibody; IK1 antibody; IKAROS antibody; LYF1 antibody; LyF-1 antibody; PPP1R92 antibody; PRO0758 antibody; ZNFN1A1 antibody; DNA-binding protein Ikaros antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human IKZF1 (NP_001207694.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - IKZF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IKZF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.
Immunofluorescence - IKZF1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using IKZF1 antibody . Blue: DAPI for nuclear staining.
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Background
Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"