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Product Name
Anti-Ikaros (1A12) Mouse antibody
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Description
Ikaros (1A12) Mouse monoclonal antibody
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Tested applications
WB, IP
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Species reactivity
Human
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Alternative names
CLL associated antigen KW 6;DNA-binding protein Ikaros;hIk 1;hIk-1;Hs.54452;IK1;Ikaros (zinc finger protein);IKAROS;IKAROS family zinc finger 1 (Ikaros);Ikaros family zinc finger protein 1;Ikzf1;IKZF1_HUMAN;LYF1;Lymphoid transcription factor LyF-1;PRO0758 antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human Ikaros(C-terminus) protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Immunoprecipitation analysis of Hela cell lysate using Ikaros (C-terminus) mouse mAb.
Western blot detection of Ikaros(C-terminus) in Ramos and Jurkat cell lysates using Ikaros(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 58KDa.Observed band size: 58KDa.
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Background
Swiss-Prot Acc.Q13422.Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells.
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