-
Product Name
IGFBP5 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to IGFBP5
-
Tested applications
WB
-
Species reactivity
Human, Mouse
-
Alternative names
IGFBP5 antibody; IBP5 antibody; insulin-like growth factor-binding protein 5 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-272 of human IGFBP5 (NP_000590.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
-
Validations
Western blot - IGFBP5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IGFBP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
-
Background
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"