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Product Name
IGFBP5 Polyclonal Antibody
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Description
Polyclonal antibody to IGFBP5
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
IGFBP5 antibody; IBP5 antibody; insulin-like growth factor-binding protein 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 68-167 of human IGFBP5 (NP_000590.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - IGFBP5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IGFBP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - IGFBP5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using IGFBP5 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - IGFBP5 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using IGFBP5 antibody at dilution of 1:100 (40x lens).
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Background
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"