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Product Name
IGFBP5 antibody
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Description
IGFBP5 Rabbit Polyclonal antibody. Positive WB detected in SKOV-3 cells. Positive IF detected in SKOV-3 cells. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
IBP 5 antibody; IBP5 antibody; IGF binding protein 5 antibody; IGFBP 5 antibody; IGFBP5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_000599). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
WB result of Catalog No:111688 (IGFBP5 Antibody) at dilution of 1:500.
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:111688(IGFBP5 antibody) at dilution of 1:500
Immunofluorescent analysis of SKOV-3 cells using Catalog No:111688(IGFBP5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBL5.
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References
- Lai G, Zhang Y, Wang L. Differences in IGF axis-related proteins in amniotic fluid of trisomy 21 and trisomy 18 using a multiple reaction monitoring approach. Prenatal diagnosis. 34(12):1146-52. 2014.
- Chen SX, Xu XE, Wang XQ. Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation. Journal of proteomics. 110:155-71. 2014.
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