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Product Name
IFT81 antibody
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Description
IFT81 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in mouse kidney tissue, HEK-293 cells. Observed molecular weight by Western-blot: 70-80kd
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CDV 1 antibody; CDV 1R antibody; CDV1 antibody; CDV1R antibody; IFT81 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IFT81 recombinant protein (Accession Number: NM_031473). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:111672(IFT81 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human testis using Catalog No:111672(IFT81 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:111672(IFT81 antibody) at dilution of 1:50 (under 40x lens)
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Background
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"