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Product Name
IFT27 antibody
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Description
IFT27 Rabbit Polyclonal antibody. Positive WB detected in human RPE cells. Positive IHC detected in human brain tissue, human testis tissue. Positive IF detected in hTERT-RPE1 cells. Observed molecular weight by Western-blot: 20 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
IFT27 antibody; Rab like protein 4 antibody; RABL4 antibody; RAYL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IFT27 recombinant protein (Accession Number: NM_001177701). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
WB result of anti-RABL4 (Catalog No:111667) with human RPE cells (siRNA and control) by Dr. Gerald Liew, Maxence Nachury's lab.
IF result (cytoplasm and the spindle midzone stain) of anti-RABL4 (IFT27, Catalog No:111667, 1:50) with telophase hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:111667(RABL4 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:111667(RABL4 Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111667(RABL4 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:111667(RABL4 Antibody) at dilution of 1:50 (under 40x lens)
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Background
RABL4, alo named as IFT27, is a GTP-binding protein that is a core component of the intraflagellar transport complex B. It plays a role in the cargo loading of the retrograde transport machinery.
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References
- Raman M, Sergeev M, Garnaas M. Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. Nature cell biology. 17(10):1356-69. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"