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Product Name
IFT140 antibody
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Description
IFT140 Rabbit Polyclonal antibody. Positive IP detected in rat testis tissue. Positive WB detected in mouse testis tissue, HepG2 cells, rat testis tissue. Positive IF detected in hTERT-RPE1 cells. Observed molecular weight by Western-blot: 140 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
c305C8.4 antibody; c380F5.1 antibody; DKFZp564L232 antibody; FLJ10306 antibody; FLJ30571 antibody; gs114 antibody; IFT140 antibody; KIAA0590 antibody; WDTC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IFT140 recombinant protein (Accession Number: NM_014714). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IF: 1:20-1:200
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Validations
IF result (enrichment to the base (arrowhead) and tip (asterix) of cilia) of anti-IFT140 (Catalog No:111664, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim.
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:111664(IFT140 Antibody) at dilution of 1:300
IP Result of anti-IFT140 (IP:Catalog No:111664, 4ug; Detection:Catalog No:111664 1:300) with rat testis tissue lysate 4400ug.
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Background
IFT140 represents intraflagellar transport 140 homolog (Chlamydomonas)
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References
- Miller KA, Ah-Cann CJ, Welfare MF. Cauli: a mouse strain with an Ift140 mutation that results in a skeletal ciliopathy modelling Jeune syndrome. PLoS genetics. 9(8):e1003746. 2013.
- Halbritter J, Bizet AA, Schmidts M. Defects in the IFT-B component IFT172 cause Jeune and Mainzer-Saldino syndromes in humans. American journal of human genetics. 93(5):915-25. 2013.
- Wei Q, Xu Q, Zhang Y. Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes. Nature communications. 4:2750. 2013.
- Yang Y, Ran J, Liu M. CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6. Cell research. 24(11):1342-53. 2014.
- Martin CA, Ahmad I, Klingseisen A. Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. Nature genetics. 46(12):1283-92. 2014.
- Xu Y, Cao J, Huang S. Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. PloS one. 10(4):e0124378. 2015.
- Ran J, Yang Y, Li D, Liu M, Zhou J. Deacetylation of α-tubulin and cortactin is required for HDAC6 to trigger ciliary disassembly. Scientific reports. 5:12917. 2015.
- Siller SS, Burke MC, Li FQ, Takemaru K. Chibby functions to preserve normal ciliary morphology through the regulation of intraflagellar transport in airway ciliated cells. Cell cycle (Georgetown, Tex.). 14(19):3163-72. 2015.
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