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Product Name
IER3 Polyclonal Antibody
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Description
Polyclonal antibody to IER3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
IER3 antibody; DIF-2 antibody; DIF2 antibody; GLY96 antibody; IEX-1 antibody; IEX-1L antibody; IEX1 antibody; PRG1 antibody; immediate early response 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human IER3 (NP_003888.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - IER3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using IER3 Antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - IER3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse stomach using IER3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - IER3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using IER3 antibody at dilution of 1:100 (40x lens).
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Background
May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A-PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival. As a member of the NUPR1/RELB/IER3 survival pathway, may provide pancreatic ductal adenocarcinoma with remarkable resistance to cell stress, such as starvation or gemcitabine treatment.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"