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Product Name
IDH1 antibody
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Description
IDH1 Mouse Monoclonal antibody. Positive WB detected in MCF-7 cells, HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas tissue, human gliomas tissue. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
IDCD antibody; IDH antibody; IDH1 antibody; IDP antibody; IDPC antibody; NADP(+) specific ICDH antibody; Oxalosuccinate decarboxylase antibody; PICD antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of IDH1 recombinant protein (Accession Number: NM_005896). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107273(IDH1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107273(IDH1 Antibody) at dilution of 1:200 (under 10x lens)
Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107273(IDH1 Antibody) at dilution of 1:200 (under 40x lens)
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Background
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"