-
Product Name
ICT1 antibody
- Documents
-
Description
ICT1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in A2780 cells, SKOV-3 cells. Positive IP detected in SKOV-3 cells. Observed molecular weight by Western-blot: 19-24kd
-
Tested applications
ELISA, WB, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
Digestion substraction 1 antibody; DS 1 antibody; DS1 antibody; ICT1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of ICT1 recombinant protein (Accession Number: NM_001545). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IF: 1:10-1:100
-
Validations
A2780 cells were subjected to SDS PAGE followed by western blot with Catalog No:111597(ICT1 antibody) at dilution of 1:600
IP Result of anti-ICT1 (IP:Catalog No:111597, 4ug; Detection:Catalog No:111597 1:300) with SKOV-3 cells lysate 2000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:111597(ICT1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than in differentiated cells. This antibody is a rabbit polyclonal antibody raised against a full-length human ICT1 protein
-
References
- Serre V, Rozanska A, Beinat M. Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency. Biochimica et biophysica acta. 1832(8):1304-12. 2013.
- Dujeancourt L, Richter R, Chrzanowska-Lightowlers ZM, Bonnefoy N, Herbert CJ. Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. Mitochondrion. 13(6):871-80. 2013.
- Dalla Rosa I, Durigon R, Pearce SF. MPV17L2 is required for ribosome assembly in mitochondria. Nucleic acids research. 42(13):8500-15. 2014.
- Tu YT, Barrientos A. The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly. Cell reports. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"