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Product Name
ICAM3 antibody
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Description
ICAM3 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, U-937 cells. Positive FC detected in Jurkat cells. Positive IHC detected in human colon tissue. Observed molecular weight by Western-blot: 110-160 kDa, 60 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human; other species not tested.
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Alternative names
CD50 antibody; CDW50 antibody; ICAM 3 antibody; ICAM R antibody; ICAM3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ICAM3 recombinant protein (Accession Number: NM_002162). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:111591(ICAM3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human colon using Catalog No:111591(ICAM3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon using Catalog No:111591(ICAM3 antibody) at dilution of 1:100 (under 40x lens)
1X10^6 Jurkat cells were stained with 0.5ug ICAM3 antibody (Catalog No:111591, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ICAM3 represents intercellular adhesion molecule 3
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"